RT Journal Article
SR Electronic
T1 Nuclear pore-like structures in a compartmentalized bacterium
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 076430
DO 10.1101/076430
A1 Evgeny Sagulenko
A1 Amanda Nouwens
A1 Richard I. Webb
A1 Kathryn Green
A1 Benjamin Yee
A1 Garry Morgan
A1 Andrew Leis
A1 Kuo-Chang Lee
A1 Margaret K. Butler
A1 Nicholas Chia
A1 Uyen Thi Phuong Pham
A1 Stinus Lindgreen
A1 Ryan Catchpole
A1 Anthony M. Poole
A1 John A Fuerst
YR 2016
UL http://biorxiv.org/content/early/2016/09/21/076430.abstract
AB Planctomycetes are distinguished from other Bacteria by compartmentalization of cells via internal membranes, interpretation of which has been subject to recent debate regarding potential relations to Gram-negative cell structure. In our interpretation of the available data, the planctomycete Gemmata obscuriglobus contains a nuclear body compartment, and thus possesses a type of cell organization with parallels to the eukaryote nucleus. Here we show that pore-like structures occur in internal membranes of G.obscuriglobus and that they have elements structurally similar to eukaryote nuclear pores, including a basket, ring-spoke structure, and eight-fold rotational symmetry. Bioinformatic analysis of proteomic data reveals that some of the G. obscuriglobus proteins associated with pore-containing membranes possess structural domains found in eukaryote nuclear pore complexes. Moreover, immuno-gold labelling demonstrates localization of one such protein, containing a β-propeller domain, specifically to the G. obscuriglobus pore-like structures. Finding bacterial pores within internal cell membranes and with structural similarities to eukaryote nuclear pore complexes raises the dual possibilities of either hitherto undetected homology or stunning evolutionary convergence.