PT  - JOURNAL ARTICLE
AU  - Kulika Chomvong
AU  - Daniel I. Benjamin
AU  - Daniel K. Nomura
AU  - Jamie H.D. Cate
TI  - ATP homeostasis underlies optimal glucose consumption by <em>Saccharomyces cerevisiae</em>
AID  - 10.1101/076364
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 076364
4099  - http://biorxiv.org/content/early/2016/09/21/076364.short
4100  - http://biorxiv.org/content/early/2016/09/21/076364.full
AB  - Glycolysis is central to energy metabolism in most organisms, and is highly regulated to enable optimal growth. In the yeast Saccharomyces cerevisiae, feedback mechanisms that control flux through glycolysis span transcriptional control to metabolite levels in the cell. Using a cellobiose consumption pathway, we decoupled glucose sensing from carbon utilization, revealing new modular layers of control that induce ATP consumption to drive rapid carbon fermentation. Proton pumping and regulation of amino acid biosynthesis mediated by extracellular glucose sensors independently contribute to maintenance of energy homeostasis. Controlling the upper bound of cellular ATP levels may be a general mechanism used to regulate energy levels in cells, via a regulatory network that can be uncoupled from ATP concentrations under perceived starvation conditions.