TY - JOUR T1 - Using MinION nanopore sequencing to generate a <em>de novo</em> eukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red alga <em>Galdieria sulphuraria</em> strain SAG 107.79 JF - bioRxiv DO - 10.1101/076208 SP - 076208 AU - Amanda M. Davis AU - Manuela Iovinella AU - Sally James AU - Thomas Robshaw AU - Jennifer R. Dodson AU - Lorenzo Herrero-Davila AU - James H. Clark AU - Maria Agapiou AU - Simon J. McQueen-Mason AU - Gabriele Pinto AU - Claudia Ciniglia AU - James P. J. Chong AU - Peter D. Ashton AU - Seth J. Davis Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/09/20/076208.abstract N2 - We report here the de novo assembly of a eukaryotic genome using only MinION nanopore DNA sequence data by examining a novel Galdieria sulphuraria genome: strain SAG 107.79. This extremophilic red alga was targeted for full genome sequencing as we found that it could grow on a wide variety of carbon sources and could uptake several precious and rare-earth metals, which places it as an interesting biological target for disparate industrial biotechnological uses. Phylogenetic analysis clearly places this as a species of G. sulphuraria. Here we additionally show that the genome assembly generated via nanopore long read data was of a high quality with regards to low total number of contiguous DNA sequences and long length of assemblies. Collectively, the MinION platform looks to rival other competing approaches for de novo genome acquisition with available informatics tools for assembly. The genome assembly is publically released as NCBI BioProject PRJNA330791. Further work is needed to reduce small insertion-deletion errors, relative to short-read assemblies. ER -