PT  - JOURNAL ARTICLE
AU  - Mikhail G. Akimov
AU  - Alina M. Ashba
AU  - Natalia M. Gretskaya
AU  - Vladimir V. Bezuglov
TI  - N-Acyl Dopamines Induce Apoptosis in PC12 Cell Line via the O-1918-Sensitive non-CB1-non-CB2 Receptor
AID  - 10.1101/075796
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 075796
4099  - http://biorxiv.org/content/early/2016/09/19/075796.short
4100  - http://biorxiv.org/content/early/2016/09/19/075796.full
AB  - Dopamine amides of long chain fatty acids (NADA) are a family of endogenous mammalian lipids with an unknown function; they are anti-proliferative for many cancer cell lines. The aim of this study was to identify the NADA receptor responsible for cell death induction. Using PC12 cells treated with NADA in combination with various receptor blockers, or pre-treated with the known receptor agonists to down-regulate the proposed receptor, NADA were shown to induce apoptosis. This activity was blocked only by the non-CB1-non-CB2 receptor antagonist O-1918, and by the pre-treatment with the agonists of this receptor ethanolamides of palmitic and oleic acids.