%0 Journal Article %A Damien M. O’Halloran %T Phylo-Node: a molecular phylogenetic toolkit using Node.js %D 2016 %R 10.1101/075101 %J bioRxiv %P 075101 %X Background Node.js is an open-source and cross-platform environment that provides a JavaScript codebase for back-end server-side applications. JavaScript has been used to develop very fast, and user-friendly front-end tools for bioinformatic and phylogenetic analyses. However, no such toolkits are available using Node.js to conduct comprehensive molecular phylogenetic analysis.Results To address this problem, I have developed, Phylo-Node, which was developed using Node.js and provides a fast, stable, and scalable toolkit that allows the user to go from sequence retrieval to phylogeny reconstruction. Phylo-Node can execute the analysis and process the resulting outputs from sequence alignment software such as MUSCLE and Clustal Omega, the primer design software, Primer3, the best-fit modeling software, Prottest3, and the phylogeny reconstruction software, PhyML; Phylo-Node also provides very easy-to-use sequence retrieval options from NCBI. Furthermore, Phylo-Node provides simple integration and interoperation with other Node modules to develop workflows across multiple components and languages using Node inheritance patterns and a customized piping module to support the production of diverse pipelines.Conclusions Phylo-Node is open-source and freely available to all users without sign-up or login requirements. All source code and user guidelines are openly available at the GitHub repository: https://github.com/dohalloran/Phylo-Node %U https://www.biorxiv.org/content/biorxiv/early/2016/09/14/075101.full.pdf