RT Journal Article
SR Electronic
T1 A bromodomain-DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 074773
DO 10.1101/074773
A1 Thomas C.R. Miller
A1 Bernd Simon
A1 Vladimir Rybin
A1 Helga Grötsch
A1 Sandrine Curtet
A1 Saadi Khochbin
A1 Teresa Carlomagno
A1 Christoph W. Müller
YR 2016
UL http://biorxiv.org/content/early/2016/09/12/074773.abstract
AB Bromodomains are critical components of many chromatin modifying/remodeling proteins and are emerging therapeutic targets, yet how they interact with nucleosomes, rather than acetylated peptides, remains unclear. Using BRDT as a model, we characterized how the BET family of bromodomains interacts with site-specifically acetylated nucleosomes. We find that BRDT interacts with nucleosomes through its first (BD1), but not second (BD2) bromodomain, and that acetylated histone recognition by BD1 is complemented by a novel bromodomain-DNA interaction. Simultaneous DNA and histone recognition enhances BRDT's nucleosome binding affinity, specificity, and ability to localize to and compact acetylated chromatin. Conservation of DNA binding in bromodomains of BRD2, BRD3 and BRD4, indicates that bivalent nucleosome recognition is a key feature of these bromodomains and possibly others. Our results elucidate the molecular mechanism of BRDT association with nucleosomes and identify new structural features of BET bromodomains that may be targeted for therapeutic inhibition.