RT Journal Article SR Electronic T1 Single-cell transcriptome analysis of fish immune cells provides insight into the evolution of vertebrate immunity JF bioRxiv FD Cold Spring Harbor Laboratory SP 074344 DO 10.1101/074344 A1 Santiago J. Carmona A1 Sarah A. Teichmann A1 Lauren Ferreira A1 Iain C. Macaulay A1 Michael J.T. Stubbington A1 Ana Cvejic A1 David Gfeller YR 2016 UL http://biorxiv.org/content/early/2016/09/12/074344.abstract AB The immune system of vertebrate species consists of many different cell types that have distinct functional roles and are subject to different evolutionary pressures. Here, we first analysed gene conservation of all major immune cell types in human and mouse. Our results revealed higher gene turnover and faster evolution of trans-membrane proteins in NK cells compared to other immune cell populations, and especially T cells, but similar conservation of nuclear and cytoplasmic protein coding genes. To validate these findings in a distant vertebrate species, we used single-cell RNA-Sequencing of lck:GFP cells in zebrafish to obtain the first transcriptome of specific immune cell types in a non-mammalian species. Unsupervised clustering and single-cell TCR locus reconstruction identified three cell populations, T-cells, a novel type of NK-like cells and a smaller population of myeloid-like cells. Differential expression analysis uncovered new immune cell specific genes, including novel immunoglobulin-like receptors, and neofunctionalization of recently duplicated paralogs. Evolutionary analyses confirmed a higher gene turnover and lower conservation of trans-membrane proteins in NK cells compared to T cells in fish species, suggesting that this is a general property of immune cell types across all vertebrates.