PT  - JOURNAL ARTICLE
AU  - Robert C. Edgar
TI  - UCHIME2: improved chimera prediction for amplicon sequencing
AID  - 10.1101/074252
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 074252
4099  - http://biorxiv.org/content/early/2016/09/09/074252.short
4100  - http://biorxiv.org/content/early/2016/09/09/074252.full
AB  - Amplicon sequencing generates chimeric reads which can cause spurious inferences of biological variation. I describe UCHIME2, an update of the popular UCHIME chimera detection algorithm with new modes optimized for high-resolution biological sequence reconstruction (“denoising”) and other applications. I show that chimera frequency correlates inversely with divergence, that error-free chimera prediction from sequence is impossible in principle, and that UCHIME2 achieves higher detection accuracy than previous methods.