@article {Mumbach073619, author = {Maxwell R. Mumbach and Adam J. Rubin and Ryan A. Flynn and Chao Dai and Paul A. Khavari and William J. Greenleaf and Howard Y. Chang}, title = {HiChIP: Efficient and sensitive analysis of protein-directed genome architecture}, elocation-id = {073619}, year = {2016}, doi = {10.1101/073619}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.}, URL = {https://www.biorxiv.org/content/early/2016/09/08/073619}, eprint = {https://www.biorxiv.org/content/early/2016/09/08/073619.full.pdf}, journal = {bioRxiv} }