@article {Ravi Iyengar065698, author = {Bharat Ravi Iyengar and Beena Pillai and K.V. Venkatesh and Chetan J Gadgil}, title = {Dissection of the contribution of regulation mode to the properties of feedforward and feedback and gene regulatory motifs}, elocation-id = {065698}, year = {2016}, doi = {10.1101/065698}, publisher = {Cold Spring Harbor Laboratory}, abstract = {A framework enabling dissection of the effects of motif structure (feedback or feedforward), nature of the controller (mRNA or protein), and regulation mode (transcriptional, post-transcriptional or translational) on the response to a step change in the input is presented. We have used a common model for gene expression and a common parameter set. Both structures have an activating input and repressing regulatory effect. The effect of global parameter variation on steady-state gain, overshoot, peak time, and peak duration was studied. Protein mediated motifs showed higher degree of adaptation. RNA mediated motifs had a lower tendency to exhibit peaks. For both motif structures, overshoot correlated negatively with peak time. Peak duration is a concave function of peak time for all motifs.HighlightsA common modelling framework was used to compare the properties of incoherent feedforward and negative feedback motifs.Protein mediated motifs show a higher degree of adaptation.Peaking tendency is primarily dependent on nature of regulator.Overshoot decreases with peak time whereas peak duration is a concave function of peak time.Protein mediated feedbacks show a bimodal distribution of peak duration.}, URL = {https://www.biorxiv.org/content/early/2016/09/06/065698}, eprint = {https://www.biorxiv.org/content/early/2016/09/06/065698.full.pdf}, journal = {bioRxiv} }