RT Journal Article SR Electronic T1 Ultrasensitive detection of TCR hypervariable region in solid-tissue RNA-seq data JF bioRxiv FD Cold Spring Harbor Laboratory SP 073395 DO 10.1101/073395 A1 Bo Li A1 Taiwen Li A1 Binbin Wang A1 Ruoxu Dou A1 Jean-Chrisophe Pignon A1 Toni K. Choueiri A1 Sabina Signoretti A1 Jun S Liu A1 X. Shirley Liu YR 2016 UL http://biorxiv.org/content/early/2016/09/05/073395.abstract AB Characterization of tissue-infiltrating T cell repertoire is critical to understanding tumor-immune interactions and autoimmune disease etiology. We present TRUST, an open source algorithm for calling the TCR transcript hypervariable CDR3 regions using unselected RNA-seq data profiled from solid tissues. TRUST achieved high sensitivity in CDR3 calling even for samples with low sequencing depth and has demonstrated utilities in its application to large tumor cohorts.