PT - JOURNAL ARTICLE AU - FC Ingleby AU - Edward H Morrow TI - Sexual dimorphism in the <em>Drosophila</em> metabolome increases throughout development AID - 10.1101/073148 DP - 2016 Jan 01 TA - bioRxiv PG - 073148 4099 - http://biorxiv.org/content/early/2016/09/02/073148.short 4100 - http://biorxiv.org/content/early/2016/09/02/073148.full AB - The expression of sexually dimorphic phenotypes from a shared genome between males and females is a longstanding puzzle in evolutionary biology. Increasingly, research has made use of transcriptomic technology to examine the molecular basis of sexual dimorphism through gene expression studies, but even this level of detail misses the metabolic processes that ultimately link gene expression with the whole organism phenotype. We use metabolic profiling in Drosophila melanogaster to complete this missing step, with a view to examining variation in male and female metabolic profiles, or metabolomes, throughout development. We show that the metabolome varies considerably throughout larval, pupal and adult stages. We also find significant sexual dimorphism in the metabolome, although only in pupae and adults, and the extent of dimorphism tends to increase throughout development. We compare this to transcriptomic data from the same population and find that the general pattern of increasing sex differences throughout development is mirrored in RNA expression. We discuss our results in terms of the usefulness of metabolic profiling in linking genotype and phenotype to more fully understand the basis of sexually dimorphic phenotypes.