RT Journal Article
SR Electronic
T1 Contig annotation tool CAT robustly classifies assembled metagenomic contigs and long sequences
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 072868
DO 10.1101/072868
A1 Diego D. Cambuy
A1 Felipe H. Coutinho
A1 Bas E. Dutilh
YR 2016
UL http://biorxiv.org/content/early/2016/09/01/072868.abstract
AB In modern-day metagenomics, there is an increasing need for robust taxonomic annotation of long DNA sequences from unknown micro-organisms. Long metagenomic sequences may be derived from assembly of short-read metagenomes, or from long-read single molecule sequencing. Here we introduce CAT, a pipeline for robust taxonomic classification of long DNA sequences. We show that CAT correctly classifies contigs at different taxonomic levels, even in simulated metagenomic datasets that are very distantly related from the sequences in the database. CAT is implemented in Python and the required scripts can be freely downloaded from Github.