PT  - JOURNAL ARTICLE
AU  - Diego D. Cambuy
AU  - Felipe H. Coutinho
AU  - Bas E. Dutilh
TI  - Contig annotation tool CAT robustly classifies assembled metagenomic contigs and long sequences
AID  - 10.1101/072868
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 072868
4099  - http://biorxiv.org/content/early/2016/09/01/072868.short
4100  - http://biorxiv.org/content/early/2016/09/01/072868.full
AB  - In modern-day metagenomics, there is an increasing need for robust taxonomic annotation of long DNA sequences from unknown micro-organisms. Long metagenomic sequences may be derived from assembly of short-read metagenomes, or from long-read single molecule sequencing. Here we introduce CAT, a pipeline for robust taxonomic classification of long DNA sequences. We show that CAT correctly classifies contigs at different taxonomic levels, even in simulated metagenomic datasets that are very distantly related from the sequences in the database. CAT is implemented in Python and the required scripts can be freely downloaded from Github.