RT Journal Article SR Electronic T1 One-cell Doubling Evaluation by Living Arrays of Yeast, ODELAY! JF bioRxiv FD Cold Spring Harbor Laboratory SP 069724 DO 10.1101/069724 A1 Thurston Herricks A1 David J. Dilworth A1 Fred D. Mast A1 Song Li A1 Jennifer J. Smith A1 Alexander V. Ratushny A1 John D. Aitchison YR 2016 UL http://biorxiv.org/content/early/2016/08/15/069724.abstract AB Cell growth is a complex phenotype widely used in systems biology to gauge the impact of genetic and environmental perturbations. Due to the magnitude of genome-wide studies, resolution is often sacrificed in favor of throughput, creating a demand for scalable, time-resolved, quantitative methods of growth assessment. We present ODELAY (One-cell Doubling Evaluation by Living Arrays of Yeast), an automated and scalable growth analysis platform. High measurement density and single cell resolution provide a powerful tool for large-scale multiparameter growth analysis based on the modeling of microcolony expansion on solid media. Pioneered in yeast but applicable to other colony forming organisms, ODELAY extracts the three key growth parameters (lag time, doubling time, and carrying capacity) that define microcolony expansion from single cells, simultaneously permitting the assessment of population heterogeneity. The utility of ODELAY is illustrated using yeast mutants, revealing a spectrum of phenotypes arising from single and combinatorial growth parameter perturbations.