RT Journal Article
SR Electronic
T1 The Detailed 3D Multi-Loop Aggregate/Rosette Chromatin Architecture and Functional Dynamic Organization of the Human and Mouse Genomes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 064642
DO 10.1101/064642
A1 Tobias A. Knoch
A1 Malte Wachsmuth
A1 Nick Kepper
A1 Michael Lesnussa
A1 Anis Abuseiris
A1 A. M. Ali Imam
A1 Petros Kolovos
A1 Jessica Zuin
A1 Christel E. M. Kockx
A1 Rutger W. W. Brouwer
A1 Harmen J. G. van de Werken
A1 Wilfred F. J. van IJken
A1 Kerstin S. Wendt
A1 Frank G. Grosveld
YR 2016
UL http://biorxiv.org/content/early/2016/08/15/064642.abstract
AB The dynamic three-dimensional chromatin architecture of genomes and its co-evolutionary connection to its function – the storage, expression, and replication of genetic information – is still one of the central issues in biology. Here, we describe the much debated 3D-architecture of the human and mouse genomes from the nucleosomal to the megabase pair level by a novel approach combining selective high-throughput high-resolution chromosomal interaction capture (T2C), polymer simulations, and scaling analysis of the 3D-architecture and the DNA sequence: The genome is compacted into a chromatin quasi-fibre with ∼5±1 nucleosomes/11nm, folded into stable ∼30-100 kbp loops forming stable loop aggregates/rosettes connected by similar sized linkers. Minor but significant variations in the architecture are seen between cell types/functional states. The architecture and the DNA sequence show very similar fine-structured multi-scaling behaviour confirming their co-evolution and the above. This architecture, its dynamics, and accessibility balance stability and flexibility ensuring genome integrity and variation enabling gene expression/regulation by self-organization of (in)active units already in proximity. Our results agree with the heuristics of the field and allow “architectural sequencing” at a genome mechanics level to understand the inseparable systems genomic properties.