RT Journal Article
SR Electronic
T1 Plant Cysteine Oxidases are Dioxygenases that Directly Enable Arginyl Transferase-Catalyzed Arginylation of N-End Rule Targets
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 069336
DO 10.1101/069336
A1 Mark D. White
A1 Maria Klecker
A1 Richard J. Hopkinson
A1 Daan Weits
A1 Carolin Mueller
A1 Christin Naumann
A1 Rebecca O’Neill
A1 James Wickens
A1 Tom N. Grossmann
A1 Nico Dissmeyer
A1 Emily Flashman
YR 2016
UL http://biorxiv.org/content/early/2016/08/14/069336.abstract
AB Crop yield loss due to flooding is a threat to food security. Submergence-induced hypoxia in plants results in stabilisation of group VII ETHYLENE RESPONSE FACTORS (ERF-VIIs), which aid survival under the adverse conditions. ERF-VII stability is controlled by the N-end rule pathway, which proposes that ERF-VII N-terminal cysteine oxidation in normoxia enables arginylation followed by proteasomal degradation. The PLANT CYSTEINE OXIDASEs (PCOs) have been identified as catalysing this oxidation. ERF-VII stabilisation in hypoxia presumably arises from reduced PCO activity. We directly demonstrate that PCO dioxygenase activity produces Cys-sulfinic acid at the N-terminus of an ERF-VII peptide, which then undergoes efficient arginylation by an arginyl transferase (ATE1). This is the first molecular evidence showing N-terminal cysteine oxidation and arginylation by N-end rule pathway components, and the first ATE1 substrate in plants. The PCOs and ATE1 may be viable intervention targets to stabilise N-end rule substrates, including ERF-VIIs to enhance submergence tolerance in agronomy.Abbreviations PCO, plant cysteine oxidase; ATE, arginyl tRNA transferase; ERF-VII, group VII ETHYLENE RESPONSE FACTOR; 2OG, 2-oxoglutarate; NMR, nuclear magnetic resonance; Met, methionine; NME, N-terminal Met excision; Nt, N-terminal; NO, nitric oxide; HIF, hypoxia-inducible factor; PHD, prolyl hydroxylase; MALDI-MS, matrix-assisted laser desorption/ionization-mass spectrometry; LC-MS, liquid chromatography-mass spectrometry; HRE, HYPOXIA RESPONSIVE ERF; RAP, RELATED TO APETALA2; EBP, ETHYLENE RESPONSE FACTOR 72; CDO, cysteine dioxygenase; MAP, Met-aminopeptidase.