RT Journal Article SR Electronic T1 Population genomics of C. melanopterus using target gene capture data: demographic inferences and conservation perspectives JF bioRxiv FD Cold Spring Harbor Laboratory SP 068106 DO 10.1101/068106 A1 Pierpaolo Maisano Delser A1 Shannon Corrigan A1 Matthew Hale A1 Chenhong Li A1 Michel Veuille A1 Serge Planes A1 Gavin Naylor A1 Stefano Mona YR 2016 UL http://biorxiv.org/content/early/2016/08/05/068106.abstract AB Population genetics studies on non-model organisms typically involve sampling few markers from multiple individuals. Next-generation sequencing approaches open up the possibility of sampling many more markers from fewer individuals to address the same questions. Here, we applied a target gene capture method to deep sequence ∼1000 independent autosomal regions of a non-model organism, the blacktip reef shark (Carcharhinus melanopterus). We devised a sampling scheme based on the predictions of theoretical studies of metapopulations to show that sampling few individuals, but many loci, can be extremely informative to reconstruct the evolutionary history of species. We collected data from a single deme (SID) from Northern Australia and from a scattered sampling representing various locations throughout the Indian Ocean (SCD). We explored the genealogical signature of population dynamics detected from both sampling schemes using an ABC algorithm. We then contrasted these results with those obtained by fitting the data to a non-equilibrium finite island model. Both approaches supported an Nm value ∼40, consistent with philopatry in this species. Finally, we demonstrate through simulation that metapopulations exhibit greater resilience to recent changes in effective size compared to unstructured populations. We propose an empirical approach to detect recent bottlenecks based on our sampling scheme.