RT Journal Article SR Electronic T1 Single Cell Transcriptome Conservation in Cryopreserved Cells and Tissues JF bioRxiv FD Cold Spring Harbor Laboratory SP 067884 DO 10.1101/067884 A1 Amy Guillaumet-Adkins A1 Gustavo Rodríguez-Esteban A1 Elisabetta Mereu A1 Alberto Villanueva A1 August Vidal A1 Marta Gut A1 Ivo Gut A1 Holger Heyn YR 2016 UL http://biorxiv.org/content/early/2016/08/04/067884.abstract AB A variety of single cell RNA preparation procedures have been described. So far these protocols require fresh starting material, hindering complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells and so allows to disconnect time and place of sampling from subsequent processing steps. To demonstrate the potential, we sequenced single cell transcriptomes from >1,000 fresh and conserved cells. Our results confirmed that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single cell transcriptomics and could lead to a paradigm shift in future study designs.(DMSO)Dimethyl-sulfoxide(ERCC)External RNA control consortium(FBS)Fetal bovine serum(FACS)Fluorescence-activated cell sorting(GSEA)Gene set enrichment analysis(MARS-Seq)Massively parallel single-cell RNA sequencing(PAGODA)Pathway and geneset overdispersion analysis(PDOX)Patient derived orthotopic xenograft(PCA)Principal component analyses(t-SNE)T-distributed stochastic neighbor embedding(UMIs)Unique molecular identifiers