PT  - JOURNAL ARTICLE
AU  - Amy Guillaumet-Adkins
AU  - Gustavo Rodríguez-Esteban
AU  - Elisabetta Mereu
AU  - Alberto Villanueva
AU  - August Vidal
AU  - Marta Gut
AU  - Ivo Gut
AU  - Holger Heyn
TI  - Single Cell Transcriptome Conservation in Cryopreserved Cells and Tissues
AID  - 10.1101/067884
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 067884
4099  - http://biorxiv.org/content/early/2016/08/04/067884.short
4100  - http://biorxiv.org/content/early/2016/08/04/067884.full
AB  - A variety of single cell RNA preparation procedures have been described. So far these protocols require fresh starting material, hindering complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells and so allows to disconnect time and place of sampling from subsequent processing steps. To demonstrate the potential, we sequenced single cell transcriptomes from >1,000 fresh and conserved cells. Our results confirmed that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single cell transcriptomics and could lead to a paradigm shift in future study designs.(DMSO)Dimethyl-sulfoxide(ERCC)External RNA control consortium(FBS)Fetal bovine serum(FACS)Fluorescence-activated cell sorting(GSEA)Gene set enrichment analysis(MARS-Seq)Massively parallel single-cell RNA sequencing(PAGODA)Pathway and geneset overdispersion analysis(PDOX)Patient derived orthotopic xenograft(PCA)Principal component analyses(t-SNE)T-distributed stochastic neighbor embedding(UMIs)Unique molecular identifiers