RT Journal Article
SR Electronic
T1 Accelerated cell divisions drive the outgrowth of the regenerating spinal cord in axolotls
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 067785
DO 10.1101/067785
A1 Fabian Rost
A1 Aida Rodrigo Albors
A1 Vladimir Mazurov
A1 Lutz Brusch
A1 Andreas Deutsch
A1 Elly M. Tanaka
A1 Osvaldo Chara
YR 2016
UL http://biorxiv.org/content/early/2016/08/04/067785.abstract
AB Axolotls are unique in their ability to regenerate the spinal cord. However, the mechanisms that underlie this phenomenon remain poorly understood. Previously, we showed that resting stem cells in the axolotl spinal cord revert to a molecular state resembling embryonic neuroepithelial cells and functionally acquire rapid proliferative divisions during regeneration. Here we refine in space and time this increase in cell proliferation during regeneration, and identify a dynamic high-proliferation zone in the regenerating spinal cord. By tracking sparsely-labeled cells, we quantify cell influx into the regenerate. Taking a mathematical modelling approach, we integrate these quantitative biological datasets across cellular and tissue level to provide a mechanistic and quantitative understanding of regenerative spinal cord outgrowth. We find that the acceleration of the cell cycle is necessary and sufficient to drive the outgrowth of the regenerating spinal cord in axolotls.