RT Journal Article SR Electronic T1 An Atlas of Human Kinase Regulation JF bioRxiv FD Cold Spring Harbor Laboratory SP 067900 DO 10.1101/067900 A1 David Ochoa A1 Mindaugas Jonikas A1 Robert T. Lawrence A1 Bachir El Debs A1 Joel Selkrig A1 Athanasios Typas A1 Judit Villén A1 Silvia Santos A1 Pedro Beltrao YR 2016 UL http://biorxiv.org/content/early/2016/08/04/067900.abstract AB The coordinated regulation of protein kinases is a rapid mechanism that integrates diverse cues and swiftly determines appropriate cellular responses. However, our understanding of cellular decision-making has been limited by the small number of simultaneously monitored phospho-regulatory events. Here, we have estimated changes in activity in 215 human kinases in 399 conditions from a compilation of nearly 3 million phosphopeptide quantifications. This atlas identifies commonly regulated kinases as those that are central in the signaling network and defines the logic relationships between kinase pairs. Co-regulation along the conditions predicts kinase-complex and kinase-substrate associations. Additionally, the kinase regulation profile acts as a molecular fingerprint to identify related and opposing signaling states. Using this atlas, we identified essential mediators of stem cell differentiation, modulators of Salmonella infection and new targets of AKT1. This provides a global view of human phosphorylation-based signaling and the necessary context to better understand kinase driven decision-making.