RT Journal Article
SR Electronic
T1 SRST2: Rapid genomic surveillance for public health and hospital microbiology labs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 006627
DO 10.1101/006627
A1 Michael Inouye
A1 Harriet Dashnow
A1 Lesley Raven
A1 Mark B. Schultz
A1 Bernard J. Pope
A1 Takehiro Tomita
A1 Justin Zobel
A1 Kathryn E. Holt
YR 2014
UL http://biorxiv.org/content/early/2014/07/28/006627.abstract
AB Rapid molecular typing of bacterial pathogens is critical for public health epidemiology, surveillance and infection control, yet routine use of whole genome sequencing (WGS) for these purposes poses significant challenges. Here we present SRST2, a read mapping-based tool for fast and accurate detection of genes, alleles and multi-locus sequence types (MLST) from WGS data. Using >900 genomes from common pathogens, we show SRST2 is highly accurate and outperforms assembly-based methods in terms of both gene detection and allele assignment. Here we have demonstrated the use of SRST2 for microbial genome surveillance in a variety of public health and hospital settings. In the face of rising threats of antimicrobial resistance and emerging virulence amongst bacterial pathogens, SRST2 represents a powerful tool for rapidly extracting clinically useful information from raw WGS data. Source code is available from http://katholt.github.io/srst2/.