RT Journal Article
SR Electronic
T1 Data mining of Gene expression profiles of Saccharomyces cerevisiae in response to mild heat stress response
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 007468
DO 10.1101/007468
A1 Kaiyuan Ji
A1 Wenli Ma
A1 Wenling Zheng
YR 2014
UL http://biorxiv.org/content/early/2014/07/25/007468.abstract
AB We chose yeast as a model organism to explore how eukaryotic cells respond to heat stress. This study provides details on the way yeast responds to temperature changes and is therefore an empirical reference for basic cell research and industrial fermentation of yeast. We use the Qlucore Omics Explorer (QOE) bioinformatics software to analyze the gene expression profiles of the heat stress from Gene Expression Omnibus (GEO). Genes and their expression are listed in heat maps, and the gene function is analyzed against the biological processes and pathways. We can find that the expression of genes changed over time after heat stress. Gene expression changed rapidly from 0 min to 60 min after heat shock, and gene expression stabilized between 60 min to 360 min. The yeast cells begin to adjust themselves to the high temperatures in terms of the level of gene expression at about 60 min. In all of the involved pathways and biological processes, those related to ribosome and nucleic acid metabolism declined in about 15–30 min and those related to starch and sucrose increased in the same time frame. Temperature can be a simple way to control the biological processes and pathways of cell.