RT Journal Article SR Electronic T1 MethFlowVM: a virtual machine for the integral analysis of bisulfite sequencing data JF bioRxiv FD Cold Spring Harbor Laboratory SP 066795 DO 10.1101/066795 A1 Ricardo Lebrón A1 Guillermo Barturen A1 Cristina Gómez-Martín A1 José L. Oliver A1 Michael Hackenberg YR 2016 UL http://biorxiv.org/content/early/2016/07/31/066795.abstract AB The analysis of whole genome DNA methylation patterns is an important first step towards the understanding on how DNA methylation is involved in the regulation of gene expression and genome stability.Previously, we published MethylExtract, a program for DNA methylation profiling and genotyping from the same sample. Over the last years we developed it further into a methylation analysis pipeline that allows to take full advantage of novel genome assembly models. The result is a new pipeline termed MethFlow which permits both, profiling of methylation levels and differential methylation analysis.Frequently DNA methylation research is carried out in the biomedical field, where privacy issues play an important role. Therefore we implemented the pipeline into a virtual machine termed MethFlowVM which shares with a web-server its user-friendliness however, the decisive advantage is that the sequencing data does not leave the user desktop or server and therefore no privacy issues do exist.The virtual machine is available at: http://bioinfo2.uqr.es:8080/MethFlow/