RT Journal Article SR Electronic T1 Transient protein-protein interactions perturb E.coli metabolome and cause gene dosage toxicity JF bioRxiv FD Cold Spring Harbor Laboratory SP 066373 DO 10.1101/066373 A1 Sanchari Bhattacharyya A1 Shimon Bershtein A1 Jin Yan A1 Tijda Argun A1 Amy I. Gilson A1 Sunia Trauger A1 Eugene I. Shakhnovich YR 2016 UL http://biorxiv.org/content/early/2016/07/27/066373.abstract AB Several genes exhibit gene dosage toxicity yet its molecular underpinnings remain unknown. Here we demonstrate that overexpression of DHFR in E. coli causes toxic metabolic imbalance triggered by interactions with several enzymes involved in 1-carbon metabolism, in particular GlyA and PurH. DHFR overexpression partially inhibits activity of these enzymes, but at physiological concentrations, PurH-DHFR interaction enhances catalytic efficiency of DHFR, implying a functional interaction in vivo. Surprisingly, overexpression of orthologous DHFRs from other bacterial species caused minimal metabolic and fitness perturbations, despite pulling out more interacting partners than overexpressed endogenous DHFR. Orthologous DHFRs were less potent in inhibiting E. coli GlyA and PurH, or gaining a catalytic improvement upon interaction with PurH, indicating a partial loss of interaction specificity due to evolutionary divergence. This study shows how protein overexpression perturbs a dynamic network of weak yet potentially functional PPI with consequences for the metabolic state of cells and their fitness.