PT  - JOURNAL ARTICLE
AU  - Caleb N. Fischer
AU  - Eric Trautman
AU  - Jason M. Crawford
AU  - Eric V. Stabb
AU  - Nichole A. Broderick
AU  - Jo Handelsman
TI  - Metabolite exchange within the microbiome produces compounds that influence <em>Drosophila</em> behavior
AID  - 10.1101/066035
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 066035
4099  - http://biorxiv.org/content/early/2016/07/27/066035.short
4100  - http://biorxiv.org/content/early/2016/07/27/066035.full
AB  - Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however current understanding of host-microbiome interactions is derived mostly from studies in which is it is difficult to elucidate microbe-microbe interactions. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Drosophila olfactory and egg-laying behaviors differently than individual members. Drosophila prefers a Saccharomyces-Acetobacter co-culture to the same microorganisms grown individually and then mixed, a response mainly due to the conserved olfactory receptor, Or42b. Acetobacter metabolism of Saccharomyces-derived ethanol was necessary, and acetate and its metabolic derivatives were sufficient, for co-culture preference. Preference correlated with three emergent co-culture properties: ethanol catabolism, a distinct volatile emission profile, and yeast population decline. We describe a molecular mechanism by which a microbial community affects animal behavior. Our results support a model whereby emergent metabolites signal Drosophila to acquire its preferred multispecies microbiome.