@article {Fischer066035, author = {Caleb N. Fischer and Eric Trautman and Jason M. Crawford and Eric V. Stabb and Nichole A. Broderick and Jo Handelsman}, title = {Metabolite exchange within the microbiome produces compounds that influence Drosophila behavior}, elocation-id = {066035}, year = {2016}, doi = {10.1101/066035}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however current understanding of host-microbiome interactions is derived mostly from studies in which is it is difficult to elucidate microbe-microbe interactions. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Drosophila olfactory and egg-laying behaviors differently than individual members. Drosophila prefers a Saccharomyces-Acetobacter co-culture to the same microorganisms grown individually and then mixed, a response mainly due to the conserved olfactory receptor, Or42b. Acetobacter metabolism of Saccharomyces-derived ethanol was necessary, and acetate and its metabolic derivatives were sufficient, for co-culture preference. Preference correlated with three emergent co-culture properties: ethanol catabolism, a distinct volatile emission profile, and yeast population decline. We describe a molecular mechanism by which a microbial community affects animal behavior. Our results support a model whereby emergent metabolites signal Drosophila to acquire its preferred multispecies microbiome.}, URL = {https://www.biorxiv.org/content/early/2016/07/27/066035}, eprint = {https://www.biorxiv.org/content/early/2016/07/27/066035.full.pdf}, journal = {bioRxiv} }