PT  - JOURNAL ARTICLE
AU  - Z. Parackova
AU  - J. Kayserova
AU  - A. Sediva
TI  - Effect of sampling media and culture conditions on dendritic cell generation: ion depleted plasma negatively influences the survival of monocyte-derived dendritic cells
AID  - 10.1101/066233
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 066233
4099  - http://biorxiv.org/content/early/2016/07/27/066233.short
4100  - http://biorxiv.org/content/early/2016/07/27/066233.full
AB  - EDTAethylenediaminetetraacetic acidFBSfetal bovine serumMFImean fluorescence intensitymoDCmonocyte-derived dendritic cellsPBMCperipheral blood mononuclear cellsAutologous serum or plasma are often used as a supplementation in culture media for autoimmune disease studies. They contain many biologically and immunologically active molecules that might be involved in the development of these diseases. We examined the effect of different cultivating media supplemented with autologous serum and two types of plasma obtained from heparin and EDTA coated tubes on monocyte-derived dendritic cells viability. Our results show that medium with plasma from EDTA coated tubes is unsuitable for cultivation of myeloid cells due to the absence of bivalent ions and that the type of sampling tubes used for blood collection might be critical for successful experiment.