RT Journal Article SR Electronic T1 Construction of thousands of single cell genome sequencing libraries using combinatorial indexing JF bioRxiv FD Cold Spring Harbor Laboratory SP 065482 DO 10.1101/065482 A1 Sarah A. Vitak A1 Kristof A. Torkenczy A1 Jimi L. Rosenkrantz A1 Andrew J. Fields A1 Lena Christiansen A1 Melissa H. Wong A1 Lucia Carbone A1 Frank J. Steemers A1 Andrew Adey YR 2016 UL http://biorxiv.org/content/early/2016/07/23/065482.abstract AB Single cell genome sequencing has proven to be a valuable tool for the detection of somatic variation, particularly in the context of tumor evolution and neuronal heterogeneity. Current technologies suffer from high per-cell library construction costs which restrict the number of cells that can be assessed, thus imposing limitations on the ability to quantitatively measure genomic heterogeneity within a tissue. Here, we present Single cell Combinatorial Indexed Sequencing (SCI-seq) as a means of simultaneously generating thousands of low-pass single cell libraries for the purpose of somatic copy number variant detection. In total, we constructed libraries for 16,698 single cells from a combination of cultured cell lines, frontal cortex tissue from Macaca mulatta, and two human adenocarcinomas. This novel technology provides the opportunity for low-cost, deep characterization of somatic copy number variation in single cells, providing a foundational knowledge across both healthy and diseased tissues.