RT Journal Article
SR Electronic
T1 A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically Encoded Fluorescent Amino Acids
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 065227
DO 10.1101/065227
A1 Ximena Steinberg
A1 Jason Galpin
A1 Gibran Nasir
A1 Jose Sepulveda-Ugarte
A1 Romina V. Sepúlveda
A1 Fernando Gonzalez-Nilo
A1 Leon D. Islas
A1 Christopher A. Ahern
A1 Sebastian Brauchi
YR 2016
UL http://biorxiv.org/content/early/2016/07/22/065227.abstract
AB The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of an orthogonal aminoacyltRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells.