PT  - JOURNAL ARTICLE
AU  - Ximena Steinberg
AU  - Jason Galpin
AU  - Gibran Nasir
AU  - Jose Sepulveda-Ugarte
AU  - Romina V. Sepúlveda
AU  - Fernando Gonzalez-Nilo
AU  - Leon D. Islas
AU  - Christopher A. Ahern
AU  - Sebastian Brauchi
TI  - A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically Encoded Fluorescent Amino Acids
AID  - 10.1101/065227
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 065227
4099  - http://biorxiv.org/content/early/2016/07/22/065227.short
4100  - http://biorxiv.org/content/early/2016/07/22/065227.full
AB  - The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of an orthogonal aminoacyltRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells.