%0 Journal Article
%A Ryan Andres
%A Viktoriya Coneva
%A Margaret H. Frank
%A John R. Tuttle
%A Sang-Won Han
%A Luis Fernando Samayoa
%A Baljinder Kaur
%A Linglong Zhu
%A Hui Fang
%A Daryl Bowman
%A Marcela Rojas-Pierce
%A Candace H. Haigler
%A Don C. Jones
%A James B. Holland
%A Daniel H. Chitwood
%A Vasu Kuraparthy
%T Modifications to a LATE MERISTEM IDENTITY-1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.)
%D 2016
%R 10.1101/062612
%J bioRxiv
%P 062612
%X Manipulation of cotton canopy architecture offers the potential to maximize yield while minimizing inputs. Here we show that the major leaf shapes of cotton at the L-D1 locus are controlled by a Late Meristem Identity1-D1b (GhLMI1-D1b) gene which encodes a HD-Zip transcription factor. Okra leaf GhLMI1-D1b has a 133-bp tandem duplication in the promoter, correlated with elevated expression, while an 8-bp deletion in the third exon of normal leaf GhLMI1-D1b causes a frame-shifted and truncated coding sequence. Virus-induced gene silencing (VIGS) of GhLMI1-D1b in an okra variety was sufficient to induce normal leaf formation. An intermediate leaf shape allele, sub-okra, lacks both the promoter duplication and the exonic deletion. Our results indicate that sub-okra is the ancestral leaf shape of tetraploid cotton and normal is a derived mutant allele that came to predominate and define the leaf shape of cultivated cotton.
%U https://www.biorxiv.org/content/biorxiv/early/2016/07/07/062612.full.pdf