RT Journal Article SR Electronic T1 Comments on: “A comprehensive repertoire of tRNA-derived fragments in prostate cancer” JF bioRxiv FD Cold Spring Harbor Laboratory SP 061572 DO 10.1101/061572 A1 Rogan Magee A1 Phillipe Loher A1 Aristeidis G. Telonis A1 Yohei Kirino A1 Isidore Rigoutsos YR 2016 UL http://biorxiv.org/content/early/2016/07/07/061572.abstract AB We evaluated the deep-sequencing (RNA-seq) data from human prostate tissue that were reported in [1] and the tRNA-derived fragments described in the original analysis. Our study of the same RNA-seq datasets reveals a considerably different pool of tRNA fragments, many of them with higher abundances than the fragments reported in [1]. We also evaluated the q-PCR approach proposed in [1]. As the approach lacks 5’-endpoint specificity, it will not estimate correctly the abundance of many of the tRFs that are present in the sampled RNA populations from human prostate tissue.