@article {Magee061572, author = {Rogan Magee and Phillipe Loher and Aristeidis G. Telonis and Yohei Kirino and Isidore Rigoutsos}, title = {Comments on: {\textquotedblleft}A comprehensive repertoire of tRNA-derived fragments in prostate cancer{\textquotedblright}}, elocation-id = {061572}, year = {2016}, doi = {10.1101/061572}, publisher = {Cold Spring Harbor Laboratory}, abstract = {We evaluated the deep-sequencing (RNA-seq) data from human prostate tissue that were reported in [1] and the tRNA-derived fragments described in the original analysis. Our study of the same RNA-seq datasets reveals a considerably different pool of tRNA fragments, many of them with higher abundances than the fragments reported in [1]. We also evaluated the q-PCR approach proposed in [1]. As the approach lacks 5{\textquoteright}-endpoint specificity, it will not estimate correctly the abundance of many of the tRFs that are present in the sampled RNA populations from human prostate tissue.}, URL = {https://www.biorxiv.org/content/early/2016/07/07/061572}, eprint = {https://www.biorxiv.org/content/early/2016/07/07/061572.full.pdf}, journal = {bioRxiv} }