RT Journal Article SR Electronic T1 Forward genetic screen of human transposase genomic rearrangements JF bioRxiv FD Cold Spring Harbor Laboratory SP 061770 DO 10.1101/061770 A1 Anton G. Henssen A1 Eileen Jiang A1 Jiali Zhuang A1 Luca Pinello A1 Nicholas D. Socci A1 Richard Koche A1 Mithat Gonen A1 Camila M. Villasante A1 Scott A. Armstrong A1 Daniel E. Bauer A1 Zhiping Weng A1 Alex Kentsis YR 2016 UL http://biorxiv.org/content/early/2016/07/01/061770.abstract AB Background. Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates. Results. To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates. Conclusions. The discovered PSS motifs and high-throughput forward chemical genomic screening approach should prove useful for the elucidation of endogenous genome remodeling activities of PGBD5 and other domesticated human DNA transposases and recombinases.