RT Journal Article
SR Electronic
T1 Multi-locus analysis of genomic time series data from experimental evolution
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 006734
DO 10.1101/006734
A1 Jonathan Terhorst
A1 Yun S. Song
YR 2014
UL http://biorxiv.org/content/early/2014/06/28/006734.abstract
AB Genomic time series data generated by evolve-and-resequence (E&R) experiments offer a powerful window into the mechanisms that drive evolution. However, standard population genetic inference procedures do not account for sampling serially over time, and new methods are needed to make full use of modern experimental evolution data. To address this problem, we develop a Gaussian process approximation to the multi-locus Wright-Fisher process with selection over a time course of tens of generations. The mean and covariance structure of the Gaussian process are obtained by computing the corresponding moments in discrete-time Wright-Fisher models conditioned on the presence of a linked selected site. This enables our method to account for the effects of linkage and selection, both along the genome and across sampled time points, in an approximate but principled manner. Using simulated data, we demonstrate the power of our method to correctly detect, locate and estimate the fitness of a selected allele from among several linked sites. We also study how this power changes for different values of selection strength, initial haplotypic diversity, population size, sampling frequency, experimental duration, number of replicates, and sequencing coverage depth. In addition to providing quantitative estimates of selection parameters from experimental evolution data, our model can be used by practitioners to design E&R experiments with requisite power. Finally, we explore how our likelihood-based approach can be used to infer other model parameters, including effective population size and recombination rate, and discuss extensions to more complex models.