PT - JOURNAL ARTICLE AU - Tony Hoang AU - Dhruv S. Patel AU - Ken Halvorsen TI - A wireless centrifuge force microscope (CFM) enables multiplexed single-molecule experiments in a commercial centrifuge AID - 10.1101/060269 DP - 2016 Jan 01 TA - bioRxiv PG - 060269 4099 - http://biorxiv.org/content/early/2016/06/22/060269.short 4100 - http://biorxiv.org/content/early/2016/06/22/060269.full AB - The centrifuge force microscope (CFM) was recently introduced as a platform for massively parallel single-molecule manipulation and analysis. Here we developed a low-cost and selfcontained CFM module that works directly within a commercial centrifuge, greatly improving accessibility and ease of use. Our instrument incorporates research grade video microscopy, a power source, a computer, and wireless transmission capability to simultaneously monitor many individually tethered microspheres. We validated the instrument by performing single-molecule force shearing of short DNA duplexes. For a 7 bp duplex, we collected over 1000 statistics of force dependent shearing kinetics from 2 pN to 12 pN with dissociation times in the range of 10-100 seconds. We extended the measurement to a 10 bp duplex, applying a 12 pN force clamp and directly observing single-molecule dissociation over an 85 minute experiment. Our new CFM module facilitates simple and inexpensive experiments that dramatically improve access to singlemolecule analysis.