RT Journal Article
SR Electronic
T1 Heterologous protection against Asian Zika virus challenge in rhesus macaques
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 059592
DO 10.1101/059592
A1 Matthew T. Aliota
A1 Dawn M. Dudley
A1 Christina M. Newman
A1 Emma L. Mohr
A1 Dane D. Gellerup
A1 Meghan E. Breitbach
A1 Connor R. Buechler
A1 Mustafa N. Rasheed
A1 Mariel S. Mohns
A1 Andrea M. Weiler
A1 Gabrielle L. Barry
A1 Kim L. Weisgrau
A1 Josh A. Eudailey
A1 Eva G. Rakasz
A1 Logan J. Vosler
A1 Jennifer Post
A1 Saverio Capuano III
A1 Thaddeus G. Golos
A1 M. Anthony Moody
A1 Sallie R. Permar
A1 Jorge E. Osorio
A1 Thomas C. Friedrich
A1 Shelby L. O’Connor
A1 David H. O’Connor
YR 2016
UL http://biorxiv.org/content/early/2016/06/17/059592.abstract
AB Zika virus (ZIKV) isolates are genetically diverse, but belong to two recognized lineages, termed “African” and “Asian.” Asian ZIKV infection during pregnancy causes fetal abnormalities including microcephaly. Developing an effective preventative Zika virus vaccine that protects pregnant women is essential for minimizing fetal abnormalities; at least 18 groups are developing ZIKV vaccines (Hayden, 2016). The genetic and antigenic variability of many RNA viruses limits the effectiveness of vaccines, and the degree to which immunity against one ZIKV strain could provide protection against another is unknown. Here we show that rhesus macaques infected with East African ZIKV strain MR766 are completely protected from subsequent infection with heterologous Asian ZIKV. MR766 is more genetically divergent from all known Asian ZIKV strains than Asian ZIKV strains are from one another. Therefore, ZIKV strain selection is unlikely to compromise vaccine effectiveness.HighlightsAfrican Zika virus (ZIKV) strain MR766 productively infects macaques (68 characters) Immunity elicited by MR766 protects macaques against heterologous Asian ZIKV (77 characters)In vivo restoration of a putative N-linked glycosylation site in MR766 (70 characters)Immunogen selection is unlikely to adversely affect the breadth of vaccine protection (85 characters)eTOC An effective Zika virus vaccine is needed to prevent infection-associated fetal abnormalities. Macaques whose immune responses are primed by infection with East African ZIKV are completely protected from reinfection with heterologous Asian ZIKV. Any Asian ZIKV immunogen that protects against homologous challenge will likely confer protection against all other Asian ZIKV strains.