RT Journal Article
SR Electronic
T1 In Situ Replication Rates for Uncultivated Bacteria in Microbial Communities
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 057992
DO 10.1101/057992
A1 Christopher T. Brown
A1 Matthew R. Olm
A1 Brian C. Thomas
A1 Jillian F. Banfield
YR 2016
UL http://biorxiv.org/content/early/2016/06/16/057992.abstract
AB Culture-independent microbiome studies have revolutionized our understanding of the complexity and metabolic potential of microbial communities, but information about in situ growth rates has been lacking. Here, we show that bacterial replication rates can be determined using genome-resolved metagenomics without requirement for complete genome sequences. In human infants, we detected elevated microbial replication rates following administration of antibiotics, and bacterial growth rate anomalies prior to the onset of necrotizing enterocolitis. We studied microorganisms in subsurface communities and determined that a diverse group of groundwater-associated bacteria typically exhibit slow growth rates, despite significant changes in geochemical conditions. All microbiome studies will be advanced by measurements of replication rates that can identify actively growing populations, track organism responses to changing conditions, and provide growth rate information needed for modeling.