TY - JOUR T1 - Actomyosin Dynamics Determine the Extension and Retraction of Filopodia on Neuronal Dendrites JF - bioRxiv DO - 10.1101/057919 SP - 057919 AU - Olena O. Marchenko AU - Sulagna Das AU - Ji Yu AU - Igor L. Novak AU - Vladimir I. Rodionov AU - Nadia Efimova AU - Tatiana Svitkina AU - Charles W. Wolgemuth AU - Leslie M. Loew Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/06/16/057919.abstract N2 - Impact Statement In this study, using a combination of computational and experimental approaches we show that a complex dynamic behavior of dendritic filopodia that is essential for synaptogenesis is explained by an interplay among forces generated by actin retrograde flow, myosin contractility, and substrate adhesion.Abstract Dendritic filopodia are actin-filled dynamic subcellular structures that sprout on neuronal dendrites during neurogenesis. The exploratory motion of the filopodia is crucial for synaptogenesis but the underlying mechanisms are poorly understood. To study the filopodial motility, we collected and analyzed image data on filopodia in cultured rat hippocampal neurons. We hypothesized that mechanical feedback among the actin retrograde flow, myosin activity and substrate adhesion gives rise to various filopodial behaviors. We have formulated a minimal one-dimensional partial differential equation model that reproduced the range of observed motility. To validate our model, we systematically manipulated experimental correlates of parameters in the model: substrate adhesion strength, actin polymerization rate, myosin contractility and the integrity of the putative microtubule-based barrier at the filopodium base. The model predicts the response of the system to each of these experimental perturbations, supporting the hypothesis that our actomyosin-driven mechanism controls dendritic filopodia dynamics. ER -