TY - JOUR T1 - Networks that Link Cytoskeletal Regulators and Diaphragm Proteins Underpin Filtration Function in <em>Drosophila</em> Nephrocytes JF - bioRxiv DO - 10.1101/056911 SP - 056911 AU - Simi Muraleedharan AU - Helen Skaer AU - Maneesha S. Inamdar Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/06/14/056911.abstract N2 - Insect nephrocytes provide a valuable model for kidney disease, as they are structurally and functionally homologous to mammalian kidney podocytes. They possess an exceptional macromolecular assembly, the nephrocyte diaphragm (ND), which serves as a filtration barrier and helps maintain tissue homeostasis by filtering out wastes and toxic products. However, the nephrocyte architecture and elements that maintain the ND are not understood. We show that Drosophila nephrocytes have a unique cytoplasmic cluster of F-actin, which is maintained by the microtubule cytoskeleton and Rho-GTPases. A balance of Racl and Cdc42 activity as well as proper microtubule organization is required for positioning the actin cluster. Further, ND proteins Sns and Duf also localize to this cluster and regulate organization of the actin and microtubule cytoskeleton. Perturbation of any of these inter-dependent components impairs nephrocyte ultrafiltration. Thus cytoskeletal components, Rho-GTPases and ND proteins work in concert to maintain the specialized nephrocyte architecture and function. ER -