@article {Wurzbacher058628, author = {Christian Wurzbacher and Katrin Attermeyer and Marie Therese Kettner and Clara Flintrop and Norman Warthmann and Sabine Hilt and Hans-Peter Grossart and Michael T. Monaghan}, title = {Universal metabarcoding of pico- to mesoplankton reveals seasonal dynamics and a bacterial bloom}, elocation-id = {058628}, year = {2016}, doi = {10.1101/058628}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Most studies of biodiversity focus on either macroscopic or microbial communities, with little or no simultaneous study of eukaryotes and prokaryotes. We tested whether a universal metabarcoding approach could be used to study the total diversity and temporal dynamics of aquatic pico- to mesoplankton communities in a shallow temperate lake. The approach revealed significant changes in the relative abundance of eukaryotic and prokaryotic plankton communities over a period of three months. These patterns, based on sequencing reads, fit with counts using traditional methods. We also witnessed the bloom of a conditionally rare bacterial taxon belonging to Arcicella, a genus that has been largely overlooked in freshwaters. Our data demonstrate the potential of universal metabarcoding as a complement to traditional studies of plankton communities, and for long-term monitoring across a broad range of organisms.}, URL = {https://www.biorxiv.org/content/early/2016/06/13/058628}, eprint = {https://www.biorxiv.org/content/early/2016/06/13/058628.full.pdf}, journal = {bioRxiv} }