RT Journal Article
SR Electronic
T1 Differential analysis of RNA-Seq incorporating quantification uncertainty
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 058164
DO 10.1101/058164
A1 Harold Pimentel
A1 Bray Nicolas L.
A1 Suzette Puente
A1 Páll Melsted
A1 Lior Pachter
YR 2016
UL http://biorxiv.org/content/early/2016/06/10/058164.abstract
AB We describe a novel method for the differential analysis of RNA-Seq data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. The method is implemented in an interactive shiny app called sleuth that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of RNA-Seq experiments.