RT Journal Article
SR Electronic
T1 <em>NGMASTER: in Silico</em> Multi-Antigen Sequence Typing for <em>Neisseria gonorrhoeae</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 057760
DO 10.1101/057760
A1 Jason C Kwong
A1 Anders Gonçalves da Silva
A1 Kristin Dyet
A1 Deborah A Williamson
A1 Timothy P Stinear
A1 Benjamin P Howden
A1 Torsten Seemann
YR 2016
UL http://biorxiv.org/content/early/2016/06/09/057760.abstract
AB Whole-genome sequencing (WGS) provides the highest resolution analysis for comparison of bacterial isolates in public health microbiology. However, although increasingly being used routinely for some pathogens such as Listeria monocytogenes and Salmonella enterica, the use of WGS is still limited for other organisms, such as Neisseria gonorrhoeae. Multi-antigen sequence typing (NG-MAST) is the most widely performed typing method for epidemiologic surveillance of gonorrhoea. Here, we present NGMASTER – a command-line software tool for performing in silico NG-MAST on assembled genome data. NGMASTER rapidly and accurately determined the NG-MAST of 630 assembled genomes, facilitating comparisons between WGS and previously published gonorrhoea epidemiological studies. The source code and user documentation are available at https://github.com/MDU-PHL/ngmaster.