TY - JOUR T1 - Rapid, one-step generation of biallelic conditional gene knockouts JF - bioRxiv DO - 10.1101/056549 SP - 056549 AU - Amanda Andersson-Rolf AU - Roxana C. Mustata AU - Alessandra Merenda AU - Sajith Perera AU - Tiago Grego AU - Jihoon Kim AU - Katie Andrews AU - Juergen Fink AU - William C. Skarnes AU - Bon-Kyoung Koo Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/06/01/056549.abstract N2 - Loss-of-function studies are key to investigate gene function and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional gene knockouts in diploid cells by co-delivery of CRISPR/Cas9 and a universal conditional intron cassette. ER -