RT Journal Article
SR Electronic
T1 ROS signaling in cotton ovule epidermal cells is necessary for fiber initiation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 000745
DO 10.1101/000745
A1 Mingxiong Pang
A1 Nicholas Sanford
A1 Thea A Wilkins
YR 2013
UL http://biorxiv.org/content/early/2013/11/19/000745.abstract
AB Cotton (Gossypium hirsutum) fiber, an extremely elongated and thickened single cell of the seed epidermis, is the world’s most important natural and economical textile fiber. Unlike Arabidopsis leaf trichomes, fiber initials are randomly developed and frequently form in adjacent seed epidermal cells and follow no apparent pattern. Numerous publications suggested cotton fiber development shares a similar mechanism with Arabidopsis leaf trichome development. Here we show that H2O2 accumulation in cotton ovule epidermal cells by NBT staining ovules at different development stages between TM1 and N1n2, a lintless-fuzzless doubled mutant originated from TM1. In contrast, Arabidopsis and cotton leaf trichomes do not show H2O2 content. By adding DPI (H2O2 inhibitor) and SHAM (H2O2 activator) in vitro ovule cultures, we show fiber initiation directly involves with H2O2 accumulation. We propose that the directional accumulation of H2O2 in cotton ovule epidermal cell is the drive for fiber initiation, elongation.