TY - JOUR T1 - Widespread natural variation of DNA methylation within angiosperms JF - bioRxiv DO - 10.1101/045880 SP - 045880 AU - Chad E. Niederhuth AU - Adam J. Bewick AU - Lexiang Ji AU - Magdy S. Alabady AU - Kyung Do Kim AU - Qing Li AU - Nicholas A. Rohr AU - Aditi Rambani AU - John M. Burke AU - Josh A. Udall AU - Chiedozie Egesi AU - Jeremy Schmutz AU - Jane Grimwood AU - Scott A. Jackson AU - Nathan M. Springer AU - Robert J. Schmitz Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/28/045880.abstract N2 - To understand the variation in genomic patterning of DNA methylation we compared methylomes of 34 diverse angiosperm species. By analyzing whole-genome bisulfite sequencing data in a phylogenetic context it becomes clear that there is extensive variation throughout angiosperms in gene body DNA methylation, euchromatic silencing of transposons and repeats, as well as silencing of heterochromatic transposons. The Brassicaceae have reduced CHG methylation levels and also reduced or loss of CG gene body methylation. The Poaceae are characterized by a lack or reduction of heterochromatic CHH methylation and enrichment of CHH methylation in genic regions. Reduced CHH methylation levels are found in clonally propagated species, suggesting that these methods of propagation may alter the epigenomic landscape over time. These results show that DNA methylation patterns are broadly a reflection of the evolutionary and life histories of plant species. ER -