RT Journal Article
SR Electronic
T1 Validation of methods for Low-volume RNA-seq
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 006130
DO 10.1101/006130
A1 Peter A. Combs
A1 Michael B. Eisen
YR 2014
UL http://biorxiv.org/content/early/2014/06/10/006130.abstract
AB Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using existing approaches invalid. In this study, we performed a critical evaluation of several of these low-volume RNA-seq protocols, and found that they performed slightly less well in metrics of interest to us than a more standard protocol, but with at least two orders of magnitude less sample required. We also explored a simple modification to one of these protocols that, for many samples, reduced the cost of library preparation to approximately $20/sample.