TY - JOUR T1 - Odd-paired controls frequency doubling in <em>Drosophila</em> segmentation by altering the pair-rule gene regulatory network JF - bioRxiv DO - 10.1101/052241 SP - 052241 AU - Erik Clark AU - Michael Akam Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/26/052241.abstract N2 - The Drosophila embryo transiently exhibits a double segment periodicity, defined by the expression of seven "pair-rule" genes, each in a pattern of seven stripes. At gastrulation, interactions between the pair-rule genes lead to frequency doubling and the patterning of fourteen parasegment boundaries. In contrast to earlier stages of Drosophila anterior-posterior patterning, this transition is not well understood. By carefully analysing the spatiotemporal dynamics of pair-rule gene expression, we show that frequency-doubling is precipitated by multiple coordinated changes to the network of regulatory interactions between the pair-rule genes. We identify the broadly expressed but temporally patterned transcription factor, Odd-paired (Opa/Zic), as the cause of these changes, and propose a new model for the patterning of even-numbered parasegment boundaries that relies on Opa-dependent regulatory interactions. Our findings indicate that the pair-rule gene regulatory network has a temporally-modulated topology, permitting the pair-rule genes to play stage-specific patterning roles. ER -